The standardization of ghritas such as amritaprasa ghrita, brahmi ghrita, chagalyadi ghrita and phala ghrita has been studied. These ghritas are the important Ayurvedic formulations used for peri-natal care of mother and child health. Standardization of ghritas were achieved by organoleptic study, physico-chemical analysis, qualitative analysis, thin layer chromatography (TLC), UV - visible spectrophotometry and high performance liquid chromatographic (HPLC) fingerprint studies. Qualitative analysis of alcoholic extracts of all the four ghritas shows the presence of glycosides and hexane extracts shows the presence of glycosides and steroids. TLC study of ghritas was carried out in toulene-ethyl acetate solvent system. Hexane extracts of ghritas were used for UV- visible spectrophotometry and qualitative HPLC fingerprint study.